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Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Cannabinoid Receptor II.
(Peptide available as ab45941.)
Please note that this antibody is not suitable for the use with human samples.
Our Abpromise guarantee covers the use of ab45942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).Can be blocked with Cannabinoid Receptor II peptide (ab45941).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
Immunocytochemical immunofluorescence analysis of methanol-fixed HT29 human cell line, labelling cannabinoid receptor II with ab45942 at 1/50 dilution incubated for 18 hours at 4°C in 1% BSA. Fixed cells were permeabilized with 0.25% Tween. Secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 488. Counterstain is DAPI against nuclear DNA.