The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/16000.
WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHeterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
Tissue specificityPreferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Post-translational modificationsConstitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
Cellular localizationCell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.