Recombinant
RabMAb

Anti-CAP2 antibody [EPR6377] (ab124692)

Overview

  • Product name
    Anti-CAP2 antibody [EPR6377]
    See all CAP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6377] to CAP2
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues from Human CAP2.

  • Positive control
    • HepG2, SH-SY5Y, Human heart lysates; permeabilized SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      May have a regulatory bifunctional role.
    • Sequence similarities
      Belongs to the CAP family.
      Contains 1 C-CAP/cofactor C-like domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810452G09Rik antibody
      • adenylate cyclase associated protein 2 antibody
      • Adenylyl cyclase associated protein 2 antibody
      • Adenylyl cyclase-associated protein 2 antibody
      • CAP 2 antibody
      • CAP adenylate cyclase associated protein 2 antibody
      • CAP adenylate cyclase-associated protein 2 antibody
      • CAP, adenylate cyclase associated protein, 2 (yeast) antibody
      • cap2 antibody
      • CAP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-CAP2 antibody [EPR6377] (ab124692) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124692, at 1/100, staining CAP2 in permeabilized SH-SY5Y cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Masugi Y  et al. Overexpression of adenylate cyclase-associated protein 2 is a novel prognostic marker in malignant melanoma. Pathol Int 65:627-34 (2015). IHC-P ; Human . Read more (PubMed: 26374196) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Duodenum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M sodium citrate buffer
    Permeabilization
    No
    Specification
    Duodenum
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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