Recombinant
RabMAb

Anti-CAP2 antibody [EPR6377] (ab124692)

Overview

  • Product name
    Anti-CAP2 antibody [EPR6377]
    See all CAP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6377] to CAP2
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues from Human CAP2.

  • Positive control
    • HepG2, SH-SY5Y, Human heart lysates; permeabilized SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      May have a regulatory bifunctional role.
    • Sequence similarities
      Belongs to the CAP family.
      Contains 1 C-CAP/cofactor C-like domain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2810452G09Rik antibody
      • adenylate cyclase associated protein 2 antibody
      • Adenylyl cyclase associated protein 2 antibody
      • Adenylyl cyclase-associated protein 2 antibody
      • CAP 2 antibody
      • CAP adenylate cyclase associated protein 2 antibody
      • CAP adenylate cyclase-associated protein 2 antibody
      • CAP, adenylate cyclase associated protein, 2 (yeast) antibody
      • cap2 antibody
      • CAP2_HUMAN antibody
      see all

    Anti-CAP2 antibody [EPR6377] images

    • All lanes : Anti-CAP2 antibody [EPR6377] (ab124692) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124692, at 1/100, staining CAP2 in permeabilized SH-SY5Y cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CAP2 antibody [EPR6377] (ab124692)

    This product has been referenced in:
    • Masugi Y  et al. Overexpression of adenylate cyclase-associated protein 2 is a novel prognostic marker in malignant melanoma. Pathol Int 65:627-34 (2015). IHC-P ; Human . Read more (PubMed: 26374196) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Duodenum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M sodium citrate buffer
    Permeabilization
    No
    Specification
    Duodenum
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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