The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
RelevanceCAP350, centrosome associated protein 350, is required for anchoring microtubules to the centrosomes and for the integrity of the microtubule network. It recruits PPARA to discrete subcellular compartments and thereby modulates PPARA activity.
Cellular localizationCentrosome. Spindle. Nucleus. Note=Associated with mitotic spindles. Nuclear, in discrete foci. Associated with intermediate filaments.