The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay regulate the transport and translation of mRNAs, modulating for instance the expression of proteins involved in synaptic plasticity in neurons. Involved in regulation of growth as erythroblasts shift from a highly proliferative state towards their terminal phase of differentiation. May be involved in apoptosis.
Tissue specificityDetected in all tissues tested with highest levels of expression in brain and spleen.
Sequence similaritiesBelongs to the caprin family. Contains 1 C1q domain.
Developmental stageExpression is highly regulated during erythroid development with increased expression at the stage of differentiation associated with the onset of global nuclear condensation and reduced cell proliferation.
Cellular localizationCytoplasm; Mitochondrion. Colocalizes with aggregated mitochondria and Mitochondrion. Cytoplasm. Expressed throughout the cytoplasm.