Overview

  • Product nameAnti-CAPS1 antibody
    See all CAPS1 primary antibodies
  • Description
    Rabbit polyclonal to CAPS1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Rat CAPS1.

    (Peptide available as ab32786.)

  • Positive control
    • ab32787 gave a positive result in rat and mouse brain tissue lysates. CAPS1 is known not to be expressed in heart (Speidel et al, J. Biol. Chem., Vol. 278, Issue 52, 52802-52809. PMID: 14530279). Mouse heart tissue lysate can be used as a negative control for ab32787.

Properties

Applications

Our Abpromise guarantee covers the use of ab32787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: 1/300.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Can be blocked with CAPS1 peptide (ab32786). Block in 5% milk


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCAPS1 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family which consists of two members, CAPS1 and CAPS2. The CAPS family proteins are involved in the secretion of different secretory substances in developing and postnatal brains. In addition CAPS1 regulates catecholamine release from neuroendocrine cells and is expressed predominantly in the brain.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • CADPS antibody
      • CADPS1 antibody
      • Calcium dependent secretion activator 1 antibody
      • Calcium-dependent activator protein for secretion 1 antibody
      • CAPS antibody
      • KIAA1121 antibody
      • rCAPS antibody
      see all

    Anti-CAPS1 antibody images

    • All lanes : Anti-CAPS1 antibody (ab32787) at 1 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate at 10 µg
      Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
      Lane 3 : Heart (Mouse) Tissue Lysate at 10 µg
      Lane 4 : Brain (Rat) Tissue Lysate with Rat CAPS1 peptide (ab32786) at 1 µg/ml
      Lane 5 : Brain (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml
      Lane 6 : Heart (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 146 kDa
      Observed band size : 146 kDa

    References for Anti-CAPS1 antibody (ab32787)

    ab32787 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (brain sections)
    Specification brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Aug 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"