Overview

  • Product name
  • Description
    Goat polyclonal to CAPZB
  • Specificity
    This antibody is expected to specific for the beta 2 isoform of capping protein, found in non-muscle cells and should notrecognise the beta 1 form.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide:

    NDLVEALKRKQQC

    , corresponding to C terminal amino acids 261-272 of Human CAPZB.

  • Positive control
    • Hela, Jurkat and Placenta lysates.
  • General notes
    GenBank Accession Number – NP_004921.

Properties

Applications

Our Abpromise guarantee covers the use of ab1338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human CAPZB peptide (ab22806).
ELISA Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-CAPZB antibody (ab1338) at 0.01 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH-3T3 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 31 kDa
  • Lanes 1 - 2 : Anti-CAPZB antibody (ab1338) at 0.1 µg/ml
    Lane 3 :

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Pig heart lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 31 kDa
  • Anti-CAPZB antibody (ab1338) + Cell lysate prepared from Hela cells at 35 µg
    Developed using the ECL technique

    Predicted band size : 31 kDa
    ab1338 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

    Primary incubated for 1 hour.

References

This product has been referenced in:
  • Chen X  et al. Identification of differentially expressed proteins between bull X and Y spermatozoa. J Proteomics : (2012). WB . Read more (PubMed: 22820535) »
  • Shen X  et al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB ; Pig . Read more (PubMed: 19299420) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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