Anti-carabin antibody (ab77625)
Key features and details
- Goat polyclonal to carabin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-carabin antibody
See all carabin primary antibodies -
Description
Goat polyclonal to carabin -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Rhesus monkey -
Immunogen
Synthetic peptide:
C-PRRKPQTRGKTFH
, corresponding to internal sequence amino acids 405-417 of Human carabin (NP_940919.1). -
Positive control
- Human spleen lysates Flow Cyt (Intra): Jurkat cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab77625 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml.
A 1 hour primary incubation is recommended for this product. Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background. |
Notes |
---|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. A 1 hour primary incubation is recommended for this product. Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background. |
Target
-
Function
Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras. -
Tissue specificity
Most abundant in spleen and peripheral blood leukocytes. -
Sequence similarities
Contains 1 Rab-GAP TBC domain. - Information by UniProt
-
Database links
- Entrez Gene: 610509 Dog
- Entrez Gene: 374403 Human
- Entrez Gene: 108995 Mouse
- Entrez Gene: 361697 Rat
- Omim: 610831 Human
- SwissProt: Q8IV04 Human
- SwissProt: Q8C9V1 Mouse
- Unigene: 534648 Human
see all -
Alternative names
- 1810062O14Rik antibody
- AI428527 antibody
- Carabin antibody
see all
Images
-
Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab77625 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
All lanes : Anti-carabin antibody (ab77625) at 2 µg/ml
Lane 1 : Human Spleen
Lane 2 : Mouse thymus
Lysates/proteins at 35 µg per lane. -
Lanes 1 & 3 : Anti-carabin antibody (ab77625) at 0.5 µg/ml
Lane 2 : Anti-carabin antibody (ab77625) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : PBM cell lysates
Lane 3 : A431 cell lysate (negative control)
Lysates/proteins at 35 µg per lane. -
Anti-carabin antibody (ab77625) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
-
Datasheet download
References (3)
ab77625 has been referenced in 3 publications.
- Villagomez FR et al. TBC1D10C is a cytoskeletal functional linker that modulates cell spreading and phagocytosis in macrophages. Sci Rep 11:20946 (2021). PubMed: 34686741
- Zhu X et al. Cardiac-Specific EPI64C Blunts Pressure Overload-Induced Cardiac Hypertrophy. Hypertension 67:866-77 (2016). PubMed: 27021007
- Schickel JN et al. Carabin deficiency in B cells increases BCR-TLR9 costimulation-induced autoimmunity. EMBO Mol Med 4:1261-75 (2012). WB ; Mouse . PubMed: 23109291