Anti-Carbohydrate sulfotransferase 4 antibody (ab28742)

Overview

  • Product nameAnti-Carbohydrate sulfotransferase 4 antibody
  • Description
    Rabbit polyclonal to Carbohydrate sulfotransferase 4
  • Specificityab28742 recognises CHST4.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    A region within synthetic peptide: FNLQSLYPLL KDPSLNLHIV HLVRDPRAVF RSRERTKGDL MIDSRIVMGQ, corresponding to internal sequence amino acids 182-231 of Human Carbohydrate sulfotransferase 4

  • Positive control
    • Jurkat cell lysate, Human lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab28742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceCarbohydrate sulfotransferase 4 is a golgi membrane protein that is involved in enzymatic synthesis, in vitro of the disulfated disaccharide unit of corneal keratan sulfate.
  • Cellular localizationGolgi Apparatus; Golgi membrane; single-pass type II membrane protein.
  • Database links
  • Alternative names
    • Carbohydrate (N acetylglucosamine 6-O) sulfotransferase 4 antibody
    • Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 3 antibody
    • GlcNAc6ST 2 antibody
    • GST 3 antibody
    • HEC GLCNAC 6 ST antibody
    • HEC GlcNAc6ST antibody
    • High endothelial cells N acetylglucosamine 6 O sulfotransferase antibody
    • L selectin ligand sulfotransferase antibody
    • LSST antibody
    • N acetylglucosamine 6 O sulfotransferase antibody
    see all

Anti-Carbohydrate sulfotransferase 4 antibody images

  • Anti-Carbohydrate sulfotransferase 4 antibody (ab28742) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,47 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Carbohydrate sulfotransferase 4 with ab28742 at 4-8µg/ml.

References for Anti-Carbohydrate sulfotransferase 4 antibody (ab28742)

ab28742 has not yet been referenced specifically in any publications.

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