Recombinant
RabMAb

Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367)

Overview

  • Product name
    Anti-Carbonic Anhydrase I antibody [EPR5193]
    See all Carbonic Anhydrase I primary antibodies
  • Description
    Rabbit monoclonal [EPR5193] to Carbonic Anhydrase I
  • Tested applications
    Suitable for: WB, ICC, IHC-Frmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1

  • Positive control
    • K562 and TF1 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5193
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC 1/1000 - 1/5000.
IHC-Fr 1/200.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    Anti-Carbonic Anhydrase I antibody [EPR5193] images

    • ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).

      See Abreview

    • All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : TF1 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa

    References for Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367)

    This product has been referenced in:

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% gel)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Treatment
    CA1-3xflag transfected
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 30 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Muller cells and Horizontal cells)
    Specification
    Retina, Muller cells and Horizontal cells
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    Submitted Jul 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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