Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367)

Overview

  • Product nameAnti-Carbonic Anhydrase I antibody [EPR5193]
    See all Carbonic Anhydrase I primary antibodies
  • Description
    Rabbit monoclonal [EPR5193] to Carbonic Anhydrase I
  • Tested applicationsSuitable for: WB, ICC, IHC-Frmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1

  • Positive control
    • K562 and TF1 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)

Properties

Applications

Our Abpromise guarantee covers the use of ab108367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC 1/1000 - 1/5000.
IHC-Fr 1/200.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    Anti-Carbonic Anhydrase I antibody [EPR5193] images

    • ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).

      See Abreview

    • All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : TF1 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa

    References for Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367)

    ab108367 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (12.5% gel)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Treatment CA1-3xflag transfected
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 30 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample Zebrafish Tissue sections (Retina, Muller cells and Horizontal cells)
    Specification Retina, Muller cells and Horizontal cells
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Ryan MacDonald

    Verified customer

    Submitted Jul 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"