Overview

  • Product name
    Anti-Carbonic Anhydrase II antibody
    See all Carbonic Anhydrase II primary antibodies
  • Description
    Rabbit polyclonal to Carbonic Anhydrase II
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Rabbit, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Carbonic Anhydrase II.

    (Peptide available as ab92423.)

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following human tissue lysates: Bone Marrow; Small Intestine; Kidney.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • Function
    Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
  • Involvement in disease
    Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
  • Sequence similarities
    Belongs to the alpha-carbonic anhydrase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 2 antibody
    • CA II antibody
    • CA-II antibody
    • Ca2 antibody
    • CAC antibody
    • CAH2_HUMAN antibody
    • CAII antibody
    • Car 2 antibody
    • Car2 antibody
    • Carbonate dehydratase II antibody
    • Carbonic anhydrase 2 antibody
    • Carbonic anhydrase B antibody
    • Carbonic anhydrase C antibody
    • Carbonic anhydrase C, formerly antibody
    • Carbonic anhydrase II antibody
    • Carbonic dehydratase antibody
    • epididymis luminal protein 76 antibody
    • Epididymis secretory protein Li 282 antibody
    • HEL-76 antibody
    • HEL-S-282 antibody
    see all

Images



  • Predicted band size : 29 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Carbonic Anhydrase II knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: HEk293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab82559 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab82559 was shown to specifically react with Carbonic Anhydrase II when Carbonic Anhydrase II knockout samples were used. Wild-type and Carbonic Anhydrase II knockout samples were subjected to SDS-PAGE. ab82559 and ab8245 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Carbonic Anhydrase II antibody (ab82559) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 1 minute

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Blocking
Sample
Cow Purified protein (bovine carbonic anhydrases from erythrocytes)
Specification
bovine carbonic anhydrases from erythrocytes
Username

Radhika Mehta

Verified customer

Submitted Oct 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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