Anti-Carbonic Anhydrase II antibody (Biotin) (ab34675)

Overview

  • Product name
    Anti-Carbonic Anhydrase II antibody (Biotin)
    See all Carbonic Anhydrase II primary antibodies
  • Description
    Rabbit polyclonal to Carbonic Anhydrase II (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, Dot blot, Immunomicroscopy, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Carbonic Anhydrase II from Human Erythrocytes.

  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% (w/v) Sodium Azide
    Constituents: 10mg/ml BSA (IgG and Protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
Immunomicroscopy
ELISA
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000. This antibody has been assayed against 1.0µg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
    • Involvement in disease
      Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
    • Sequence similarities
      Belongs to the alpha-carbonic anhydrase family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA 2 antibody
      • CA II antibody
      • CA-II antibody
      • Ca2 antibody
      • CAC antibody
      • CAH2_HUMAN antibody
      • CAII antibody
      • Car 2 antibody
      • Car2 antibody
      • Carbonate dehydratase II antibody
      • Carbonic anhydrase 2 antibody
      • Carbonic anhydrase B antibody
      • Carbonic anhydrase C antibody
      • Carbonic anhydrase C, formerly antibody
      • Carbonic anhydrase II antibody
      • Carbonic dehydratase antibody
      • epididymis luminal protein 76 antibody
      • Epididymis secretory protein Li 282 antibody
      • HEL-76 antibody
      • HEL-S-282 antibody
      see all

    References for Anti-Carbonic Anhydrase II antibody (Biotin) (ab34675)

    ab34675 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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