Anti-Carbonic Anhydrase II antibody [EPR5195] (ab124687)


  • Product nameAnti-Carbonic Anhydrase II antibody [EPR5195]
    See all Carbonic Anhydrase II primary antibodies
  • Description
    Rabbit monoclonal [EPR5195] to Carbonic Anhydrase II
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to a sequence from the C terminus of Human Carbonic Anhydrase II.

  • Positive control
    • Human fetal brain, Caco 2, 293T, and Human heart lysates; Human kidney and brain tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionEssential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
    • Involvement in diseaseDefects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
    • Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA 2 antibody
      • CA II antibody
      • CA-II antibody
      • Ca2 antibody
      • CAC antibody
      • CAH2_HUMAN antibody
      • CAII antibody
      • Car 2 antibody
      • Car2 antibody
      • Carbonate dehydratase II antibody
      • Carbonic anhydrase 2 antibody
      • Carbonic anhydrase B antibody
      • Carbonic anhydrase C antibody
      • Carbonic anhydrase C, formerly antibody
      • Carbonic anhydrase II antibody
      • Carbonic dehydratase antibody
      • epididymis luminal protein 76 antibody
      • HEL-76 antibody
      see all

    Anti-Carbonic Anhydrase II antibody [EPR5195] images

    • All lanes : Anti-Carbonic Anhydrase II antibody [EPR5195] (ab124687) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Caco 2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • ab124687, at 1/250 dilution, staining Carbonic Anhydrase II in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab124687, at 1/250 dilution, staining Carbonic Anhydrase II in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Carbonic Anhydrase II antibody [EPR5195] (ab124687)

    ab124687 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (12.5% gel)
    Sample Human Cell lysate - whole cell (293T)
    Specification 293T
    Treatment CA2-3xflag transfected
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 30 2015

    I heard back from the lab about the last two antibodies.
    For ab32362, the immunogen sequence shares 100% homology with pig.
    For ab124687, the immunogen peptide maps between amino acids 235 - 260, on the C-terminus of human CA2 (UniProt: P0091...

    Read More

    Thank you for your inquiry.

    For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

    Read More