Anti-Carbonic Anhydrase III antibody [2CA-4] (ab49111)

Overview

  • Product nameAnti-Carbonic Anhydrase III antibody [2CA-4]
    See all Carbonic Anhydrase III primary antibodies
  • Description
    Mouse monoclonal [2CA-4] to Carbonic Anhydrase III
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionReversible hydration of carbon dioxide.
  • Tissue specificityMuscle specific.
  • Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
  • Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
  • Post-translational
    modifications
    S-glutathionylated in hepatocytes under oxidative stress.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA III antibody
    • CA-III antibody
    • Ca3 antibody
    • CAH3_HUMAN antibody
    • CAIII antibody
    • Car3 antibody
    • Carbonate dehydratase III antibody
    • Carbonic anhydrase 3 antibody
    • Carbonic anhydrase III antibody
    • Carbonic anhydrase III muscle specific antibody
    • EC 4.2.1.1 antibody
    • Epididymis secretory sperm binding protein Li 167mP antibody
    • HEL S 167mP antibody
    see all

Anti-Carbonic Anhydrase III antibody [2CA-4] images

  • Anti-Carbonic Anhydrase III antibody [2CA-4] (ab49111) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 20 minutes

References for Anti-Carbonic Anhydrase III antibody [2CA-4] (ab49111)

ab49111 has not yet been referenced specifically in any publications.

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