The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Detection limit: 5ng/lane, under reducing or non-reducing conditions.
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
RelevanceCarbonic anhydrase III is one of a group of ten zinc metalloenzymes that catalyse the reversible hydration of CO2 to form HCO3-. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury.