This antibody gave a positive signal in Recombinant Carbonic anhydrase 3 protein as well as the following tissue lysates: Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
Use a concentration of 10 µg/ml.
FunctionReversible hydration of carbon dioxide.
Tissue specificityMuscle specific.
Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
Post-translational modificationsS-glutathionylated in hepatocytes under oxidative stress.
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Anti-Carbonic Anhydrase III antibody images
Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase III antibody (ab118428)
ICC/IF image of ab118428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab118428 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - Anti-Carbonic Anhydrase III antibody (ab118428)
All lanes : Anti-Carbonic Anhydrase III antibody (ab118428) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate Lane 3 : Skeletal Muscle (Rat) Tissue Lysate Lane 4 : Carbonic Anhydrase III protein (ab50779)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique