Overview

  • Product nameAnti-Carbonic Anhydrase III antibody
    See all Carbonic Anhydrase III primary antibodies
  • Description
    Mouse monoclonal to Carbonic Anhydrase III
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-261 of Human Carbonic Anhydrase III

Properties

Applications

Our Abpromise guarantee covers the use of ab54913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReversible hydration of carbon dioxide.
    • Tissue specificityMuscle specific.
    • Sequence similaritiesBelongs to the alpha-carbonic anhydrase family.
    • Developmental stageAt 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
    • Post-translational
      modifications
      S-glutathionylated in hepatocytes under oxidative stress.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA III antibody
      • CA-III antibody
      • Ca3 antibody
      • CAH3_HUMAN antibody
      • CAIII antibody
      • Car3 antibody
      • Carbonate dehydratase III antibody
      • Carbonic anhydrase 3 antibody
      • Carbonic anhydrase III antibody
      • Carbonic anhydrase III muscle specific antibody
      • EC 4.2.1.1 antibody
      • Epididymis secretory sperm binding protein Li 167mP antibody
      • HEL S 167mP antibody
      see all

    Anti-Carbonic Anhydrase III antibody images

    • Carbonic Anhydrase III antibody (ab54913) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human skeletal muscle.


    • Predicted band size : 29 kDa
      Carbonic Anhydrase III antibody (ab54913) at 1ug/lane + K-562 cell lysate at 25ug/lane.
    • All lanes : Anti-Carbonic Anhydrase III antibody (ab54913) at 1 µg/ml

      Lane 1 : Carbonic Anhydrase III protein (ab50779) at 0.1 µg
      Lane 2 : Carbonic Anhydrase III protein (ab50779) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 90 seconds

    References for Anti-Carbonic Anhydrase III antibody (ab54913)

    This product has been referenced in:

    See 1 Publication for this product

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