Recombinant Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13425] to Carbonic Anhydrase 3/CA3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425]
See all Carbonic Anhydrase 3/CA3 primary antibodies -
Description
Rabbit monoclonal [EPR13425] to Carbonic Anhydrase 3/CA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Carbonic Anhydrase 3/CA3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P07451 -
Positive control
- Human skeletal muscle, rat muscle, and mouse muscle lysates, Human skeletal muscle tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13425 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Reversible hydration of carbon dioxide. -
Tissue specificity
Muscle specific. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Developmental stage
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. -
Post-translational
modificationsS-glutathionylated in hepatocytes under oxidative stress. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 761 Human
- Entrez Gene: 12350 Mouse
- Entrez Gene: 54232 Rat
- Omim: 114750 Human
- SwissProt: P07451 Human
- SwissProt: P16015 Mouse
- SwissProt: P14141 Rat
- Unigene: 82129 Human
see all -
Alternative names
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Carbonic Anhydrase 3/CA3 with ab181358 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody [EPR13425] (ab181358) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysates
Lane 2 : Rat muscle lysates
Lane 3 : Mouse muscle lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181358 has been referenced in 1 publication.
- Bell-Temin H et al. Measuring biological age in mice using differential mass spectrometry. Aging (Albany NY) 11:1045-1061 (2019). PubMed: 30745468