Recombinant
RabMAb

Anti-Carbonic Anhydrase III antibody [EPR13425] (ab181358)

Overview

  • Product name
    Anti-Carbonic Anhydrase III antibody [EPR13425]
    See all Carbonic Anhydrase III primary antibodies
  • Description
    Rabbit monoclonal [EPR13425] to Carbonic Anhydrase III
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Carbonic Anhydrase III aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P07451

  • Positive control
    • Human skeletal muscle, rat muscle, and mouse muscle lysates, Human skeletal muscle tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Reversible hydration of carbon dioxide.
    • Tissue specificity
      Muscle specific.
    • Sequence similarities
      Belongs to the alpha-carbonic anhydrase family.
    • Developmental stage
      At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
    • Post-translational
      modifications
      S-glutathionylated in hepatocytes under oxidative stress.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CA III antibody
      • CA-III antibody
      • Ca3 antibody
      • CAH3_HUMAN antibody
      • CAIII antibody
      • Car3 antibody
      • Carbonate dehydratase III antibody
      • Carbonic anhydrase 3 antibody
      • Carbonic anhydrase III antibody
      • Carbonic anhydrase III muscle specific antibody
      • EC 4.2.1.1 antibody
      • Epididymis secretory sperm binding protein Li 167mP antibody
      • HEL S 167mP antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Carbonic Anhydrase III with ab181358 at 1/50 dilution.

    • All lanes : Anti-Carbonic Anhydrase III antibody [EPR13425] (ab181358) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysates
      Lane 2 : Rat muscle lysates
      Lane 3 : Mouse muscle lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    ab181358 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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