Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366)

Overview

  • Product name
    Anti-Carbonic Anhydrase III antibody - N-terminal
    See all Carbonic Anhydrase III primary antibodies
  • Description
    Rabbit polyclonal to Carbonic Anhydrase III - N-terminal
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the N-terminal region of Human Carbonic Anhydrase III (NP_005172), conjugated to KLH

  • Positive control
    • HepG2 cell line lysate; Mouse kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 30 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Reversible hydration of carbon dioxide.
  • Tissue specificity
    Muscle specific.
  • Sequence similarities
    Belongs to the alpha-carbonic anhydrase family.
  • Developmental stage
    At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
  • Post-translational
    modifications
    S-glutathionylated in hepatocytes under oxidative stress.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA III antibody
    • CA-III antibody
    • Ca3 antibody
    • CAH3_HUMAN antibody
    • CAIII antibody
    • Car3 antibody
    • Carbonate dehydratase III antibody
    • Carbonic anhydrase 3 antibody
    • Carbonic anhydrase III antibody
    • Carbonic anhydrase III muscle specific antibody
    • EC 4.2.1.1 antibody
    • Epididymis secretory sperm binding protein Li 167mP antibody
    • HEL S 167mP antibody
    see all

Images

  • Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) at 1/50 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) at 1/50 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ICC/IF image of ab85366 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85366, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Holland A  et al. Comparative Label-Free Mass Spectrometric Analysis of Mildly versus Severely Affected mdx Mouse Skeletal Muscles Identifies Annexin, Lamin, and Vimentin as Universal Dystrophic Markers. Molecules 20:11317-44 (2015). Read more (PubMed: 26102067) »
  • Holland A  et al. Intricate effects of primary motor neuronopathy on contractile proteins and metabolic muscle enzymes as revealed by label-free mass spectrometry. Biosci Rep 34:N/A (2014). Read more (PubMed: 24895011) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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