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ab109717 (MS789) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate carboxylesterase 1 (CES1) from liver tissue or cell samples. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated CES1 is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
All components are shipped cold. Store all components at 4°C.
|Components||1 x 96 tests|
|100X HRP Label||1 x 125µl|
|10X Blocking Buffer||1 x 10ml|
|1X Base Buffer||1 x 25ml|
|20X Wash Buffer||1 x 25ml|
|5700X 4-nitrophenol||1 x 20µl|
|CES1 antibody precoated Microtiter plate||1 unit|
|CES1 Detector antibody||1 x 125µl|
|Extraction Buffer||1 x 15ml|
|HRP Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab109717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab109717 has not yet been referenced specifically in any publications.