Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)


  • Product nameCarboxylesterase 1 (CES1) Specific Activity Assay Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeEnzyme activity
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat, Human
  • Product overview

    ab109717 (MS789) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate carboxylesterase 1 (CES1) from liver tissue or cell samples. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated CES1 is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.


  • Notes

    All components are shipped cold. Store all components at 4°C.

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformMicroplate


  • Alternative names
    • ACAT
    • Acyl coenzyme A:cholesterol acyltransferase
    • Brain carboxylesterase hBr1
    • CES1
    • Cocaine carboxylesterase
    • Egasyn
    • HMSE
    • Liver carboxylesterase 1
    • Monocyte/macrophage serine esterase
    • REH
    • Retinyl ester hydrolase
    • Serine esterase 1
    • TGH
    • Triacylglycerol hydrolase
    see all
  • Database links


Our Abpromise guarantee covers the use of ab109717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Carboxylesterase 1 (CES1) Specific Activity Assay Kit images

  • Figure 1. Using this assay the acetylcholinesterase inhibitor tacrine, previosuly shown to co-crystalize in the active site of CES1, does not inhibit the activity of the enzyme which is in agreement with the crystalization study (Bencharit et al. Chem Biol 2003; 10(4) 341-9). Troglitazone a member of the thiazolidinediones class of antidiabetic drugs, was determined to inhihibit CES1 (IC50 3µM) while other members of this class had little/no effect (Fukami et al. Drug Metab Dispos 2010; 38(12):2173-8.
  • Principle of Sandwich ELISA used in Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717). Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.


References for Carboxylesterase 1 (CES1) Specific Activity Assay Kit (ab109717)

ab109717 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your call yesterday and for sending all of this data.
The kit developers at MitoSciences have reviewed the data and agree that it looks good, and the top three points can be removed to improve the fit of the regression line...

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Thank you for your reply.

I expect the assay will still give good results with your settings, but if you do see any unexpected data, please let me know and I will be happy to help you further.

Thank you for your phone call.

My colleague expressed concerns that since the speed of your shaker is so fast, you may see your samples spread too high up the side walls, sill out of the wells, or froth. If none of these are observed, then it...

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Thank you very much for your call this morning and for your patience while I've been in touch with the scientists in the MitoSciences lab regarding this kit.

I went ahead and forwardedyour otherquestions as well, and here is the information t...

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I looked at the spectrum - 405nm is the peak absorbance, but 360nm should work particularly with the wide window indicated. This assay will visibly turn yellow, so if there is any question about whether instrumentation is working correctl...

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Thank you for your inquiry. The protocol says to measure the wavelength at 402nm every minute for up to 1 hour for a kinetic assay. But if you would like to make an endpoint reading rather than kinetic, this can be done by observing the yellow colo...

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