Anti-Carboxymethyl Lysine antibody (ab27684)
Key features and details
- Rabbit polyclonal to Carboxymethyl Lysine
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Carboxymethyl Lysine antibody
See all Carboxymethyl Lysine primary antibodies -
Description
Rabbit polyclonal to Carboxymethyl Lysine -
Host species
Rabbit -
Specificity
This antibody specifically binds to Carboxy methyl Lysine modified proteins. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Carboxy methyl Lysine conjugated to KLH.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.015% EDTA, 0.44% Sodium chloride, 30% Glycerol (glycerin, glycerine), 1.23% Sodium phosphate -
Concentration information loading...
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Purification notes
This antibody was purified by Carboxy methyl Lysine protein Sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/2500 - 1/20000.
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WB | (1) |
1/2500 - 1/20000.
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Notes |
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ELISA
1/2500 - 1/20000. |
WB
1/2500 - 1/20000. |
Target
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Relevance
N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy. -
Alternative names
- CML antibody
- N Epsilon (Carboxymethyl) Lysine antibody
Images
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All lanes : Anti-Carboxymethyl Lysine antibody (ab27684) at 1/1000 dilution (in PBST for 18 hours at 4°C)
Lane 1 : Whole tissue lysate of raw cashew nuts
Lane 2 : Whole tissue lysate of roasted cashew nuts
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit polyclonal
Developed using the ECL technique.
Observed band size: 10-12 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 2% Milk for 30 minutes at 25°C
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab27684 has been referenced in 37 publications.
- Trim WV et al. Divergent immunometabolic changes in adipose tissue and skeletal muscle with ageing in healthy humans. J Physiol 600:921-947 (2022). PubMed: 33895996
- Zenner ML et al. Advanced glycation end-products (AGEs) are lower in prostate tumor tissue and inversely related to proportion of West African ancestry. Prostate 82:306-313 (2022). PubMed: 34855273
- Nowicka N et al. The Involvement of RAGE and Its Ligands during Progression of ALS in SOD1 G93A Transgenic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35216298
- Selejan SR et al. Renal denervation reduces atrial remodeling in hypertensive rats with metabolic syndrome. Basic Res Cardiol 117:36 (2022). PubMed: 35834066
- Dubois C et al. Exposure of Caenorhabditis elegans to Dietary Nε-Carboxymethyllysine Emphasizes Endocytosis as a New Route for Intestinal Absorption of Advanced Glycation End Products. Nutrients 13:N/A (2021). PubMed: 34959950