Anti-Carboxymethyl Lysine antibody (ab27684)

Overview

  • Product nameAnti-Carboxymethyl Lysine antibodySee all Carboxymethyl Lysine primary antibodies ...
  • Description
    Rabbit polyclonal to Carboxymethyl Lysine
  • SpecificityThis antibody specifically binds to Carboxy methyl Lysine modified proteins.
  • Tested applicationsELISA, WB, Conjugation, IHC-Fr more details
  • Immunogen

    Carboxy methyl Lysine conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab27684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/2500 - 1/20000.
WB 1/2500 - 1/20000.
Conjugation Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceN epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • Alternative names
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

Anti-Carboxymethyl Lysine antibody images

  • ab27684 staining Carboxymethyl Lysine in Mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A biotin-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Carboxymethyl Lysine antibody (ab27684) at 1/1000 dilution (in PBST for 18 hours at 4°C)

    Lane 1 : Whole tissue lysate of raw cashew nuts
    Lane 2 : Whole tissue lysate of roasted cashew nuts

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit polyclonal
    developed using the ECL technique

    Observed band size : 10-12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Chris Mattison

    Blocking Step: 2% Milk for 30 minutes at 25°C

References for Anti-Carboxymethyl Lysine antibody (ab27684)

This product has been referenced in:
  • Juranek JK  et al. RAGE deficiency improves postinjury sciatic nerve regeneration in type 1 diabetic mice. Diabetes 62:931-43 (2013). IHC ; Mouse . Read more (PubMed: 23172920) »
  • Breyer V  et al. Intracellular Glycation of Nuclear DNA, Mitochondrial DNA, and Cytosolic Proteins During Senescence-like Growth Arrest. DNA Cell Biol : (2011). WB . Read more (PubMed: 21612395) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization Yes - Triton X100 0.5%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 13 2013

Thanks for your call earlier this week and for your patience. I have looked through some literature, and I think the best positive control for these markers that we will have are the human squamous cell carcinoma slides ab5107- http://www.abcam.com/Ski...

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Application Western blot
Sample Cashew nuts (Anacardiaceae) Tissue lysate - whole (whole nut)
Loading amount 50 µg
Specification whole nut
Treatment roasting
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Mr. Chris Mattison

Verified customer

Submitted Oct 18 2011

Thank you for your call yesterday and for our conversation about positive control to use with 4HNE and CML antibodies. I have searched through some literature but need a little more time to confirm that these markers will be expressed in certain skin c...

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Thank you for your enquiry and your interest in our products. This antibody is not target specific; it recognizes any proteins which have Carboxymethyl Lysine groups. It may well be that the customers detects several different proteins. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"