Anti-Carboxymethyl Lysine antibody [CML26] (ab125145)


  • Product name
    Anti-Carboxymethyl Lysine antibody [CML26]
    See all Carboxymethyl Lysine primary antibodies
  • Description
    Mouse monoclonal [CML26] to Carboxymethyl Lysine
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Carboxymethyl Lysine conjugated to KLH.

  • Positive control
    • Human cardiac tissue (intramyocardial arteries).
  • General notes
    Other isotypes maybe present.



Our Abpromise guarantee covers the use of ab125145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/50. Fix with 4% formalin.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration. Fix with 2% phosphate-buffered glutaraldehyde solution.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Relevance
    N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • Alternative names
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody


  • Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Frescas D  et al. Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody. Proc Natl Acad Sci U S A 114:E1668-E1677 (2017). WB ; Mouse . Read more (PubMed: 28193858) »
  • Lo MC  et al. Ne-(carboxymethyl) lysine-induced mitochondrial fission and mitophagy cause decreased insulin secretion from ß-cells. Am J Physiol Endocrinol Metab 309:E829-39 (2015). Read more (PubMed: 26394662) »

See all 2 Publications for this product

Customer reviews and Q&As

The protein band size of western blot depends on which protein has adducted by CML.
Carboxymethyl Lysine (CML) is an advanced glycation end product found on proteins and lipids as a result of oxidative stress and chemical glycation. CML adducts acc...

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