Anti-Carboxymethyl Lysine antibody [CMS-10] (ab30922)

Overview

  • Product nameAnti-Carboxymethyl Lysine antibody [CMS-10]
    See all Carboxymethyl Lysine primary antibodies
  • Description
    Mouse monoclonal [CMS-10] to Carboxymethyl Lysine
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    N epsilon (Carboxymethyl) Lysine (CML) conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab30922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceN epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • Alternative names
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

References for Anti-Carboxymethyl Lysine antibody [CMS-10] (ab30922)

This product has been referenced in:
  • de la Maza MP  et al. Weight increase is associated with skeletal muscle immunostaining for advanced glycation end products, receptor for advanced glycation end products, and oxidation injury. Rejuvenation Res 11:1041-8 (2008). IHC-P . Read more (PubMed: 19086911) »

See 1 Publication for this product

Product Wall

Thank you for providing those extra details. There are a few points which would warrant optimisation in order to obtain better results withthis antibody:


1. Storage


From the datasheet we recommend that this antibody be s...

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Thank you for contacting us.

We normally use heat mediated antigen retrieval in IHC-P. The extra information when we have, is always written as application note under the application. I have checked the data currently we have for this anti...

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Thank you for contacting us and relaying the problems this customer has been having with ab30922.I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and wi...

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Thank you for contacting us.

Unfortunately we have no data about the cross-reaction of this antibody with Fructolysine.

Known cross-reactivities have been shown for Glyoxal and Glycolaldehyde. The antibody is preabsorbed for CEL a...

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I'm sorry I was not clearer in my explanation. Both ab30917 and ab30922 should be suitable for you experiment. The reason why we sometimes have what seems to be duplicates within our catalogue is that in order to make sure we have a continuous supply o...

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Thank you for contacting us. The two antibodies (ab30917 and ab30922) are both mouse monoclonal antibodies directed against N Epsilon (Carboxymethyl) Lysine (CML). They have both been tested with ELISA, IHP (frozen sections and paraffin). They ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (intervertebral disc)
Specification intervertebral disc
Fixative Formaldehyde
Antigen retrieval step Enzymatic
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 %
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Verified customer

Submitted Jan 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"