Anti-Carboxymethyl Lysine antibody [NF-1G] (ab30917)

Overview

  • Product nameAnti-Carboxymethyl Lysine antibody [NF-1G]
    See all Carboxymethyl Lysine primary antibodies
  • Description
    Mouse monoclonal [NF-1G] to Carboxymethyl Lysine
  • SpecificityThis antibody is suitable for the detection of N Epsilon (Carboxymethyl) Lysine (CML) in tissues and tissue extracts.
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    N Epsilon (Carboxymethyl) Lysine (CML)-HSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab30917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceN epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • Alternative names
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

Anti-Carboxymethyl Lysine antibody [NF-1G] images

  • ab30917 staining Carboxymethyl Lysine in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and embedded in paraffin. Samples were incubated with primary antibody (1/125 in PBS + 1% BSA) for overnight at 4°C. A Biotin-conjugated secondary was used with DAB visualisation the samples were then counterstained with haematoxylin.

References for Anti-Carboxymethyl Lysine antibody [NF-1G] (ab30917)

This product has been referenced in:
  • Crisan M  et al. Expression of Advanced Glycation End-Products on Sun-Exposed and Non-Exposed Cutaneous Sites during the Ageing Process in Humans. PLoS One 8:e75003 (2013). IHC-P ; Human . Read more (PubMed: 24116020) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - whole (cortex and hippocampus)
Specification cortex and hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RM°C
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Verified customer

Submitted Mar 28 2014

I'm sorry I was not clearer in my explanation. Both ab30917 and ab30922 should be suitable for you experiment. The reason why we sometimes have what seems to be duplicates within our catalogue is that in order to make sure we have a continuous supply o...

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Thank you for contacting us. The two antibodies (ab30917 and ab30922) are both mouse monoclonal antibodies directed against N Epsilon (Carboxymethyl) Lysine (CML). They have both been tested with ELISA, IHP (frozen sections and paraffin). They ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"