Anti-Carboxypeptidase B antibody - N-terminal (ab170724)

Overview

  • Product name
    Anti-Carboxypeptidase B antibody - N-terminal
    See all Carboxypeptidase B primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase B - N-terminal
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Carboxypeptidase B aa 16-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P15086

  • Positive control
    • Hela cells; Human pancreas tissue; K562 and Hela cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab170724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/10 - 1/50.

Target

  • Tissue specificity
    Pancreas.
  • Sequence similarities
    Belongs to the peptidase M14 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carboxypeptidase B antibody
    • Carboxypeptidase B1 (tissue) antibody
    • Carboxypeptidase B1 antibody
    • CarboxypeptidaseB antibody
    • CarboxypeptidaseB1 antibody
    • CBPB1_HUMAN antibody
    • CP B1 antibody
    • CPB 1 antibody
    • CPB antibody
    • CPB1 antibody
    • DKFZp779K1333 antibody
    • Pancreas specific protein antibody
    • Pancreas-specific protein antibody
    • Pancreatic carboxypeptidase B antibody
    • Pancreatic carboxypeptidase B1 antibody
    • PASP antibody
    • PCPB antibody
    • Procarboxypeptidase B antibody
    • Procarboxypeptidase B pancreatic antibody
    • Protaminase antibody
    • Tissue carboxypeptidase B antibody
    see all

Images

  • All lanes : Anti-Carboxypeptidase B antibody - N-terminal (ab170724) at 1/100 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Hela cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 47 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Carboxypeptidase B with ab170724 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Confocal immunofluorescent analysis of HeLa cells labeling Carboxypeptidase B with ab170724 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Flow cytometric analysis of HeLa cells labeling Carboxypeptidase B with ab170724 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170724 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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