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Synthetic peptide within Human Carboxypeptidase B aa 16-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P15086
Our Abpromise guarantee covers the use of ab170724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 47 kDa.|
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||1/10 - 1/50.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Carboxypeptidase B with ab170724 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of HeLa cells labeling Carboxypeptidase B with ab170724 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HeLa cells labeling Carboxypeptidase B with ab170724 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab170724 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"