RelevanceThis protein belongs to a family of Zn-containing metallocarboxypeptidases specific to C-terminal lysine and arginine residues. It circulates in plasma as a zymogen with molecular weight of 55 kDa (401 amino acid residues; pI 5.0). Being activated by thrombin-thrombomodulin complex during blood coagulation, it exerts carboxypeptidase activity. Activated carboxypeptidase B2 removes C-terminal lysine residues from fibrin, which is necessary for plasminogen binding to fibrin. This prevents plasminogen from activation into plasmin and retards the lysis of a fibrin clot. The concentration in plasma of healthy people is 5 – 10 µg/ml. High plasma levels were found in patients with stable angina pectoris and angiographically verified coronary artery disease. Elevated concentration in blood is considered as a risk factor for venous thrombosis. A deficiency might contribute to the severity of bleeding disorders in hemophilias A and B, and decreased levels are found in chronic liver disease.
Titration curves of carboxypeptidase B2 in plasma using antibody pair; capture ab14756, 200ng/well and HRPconjugated antibody ab14757. Incubated at room temperature.
Western blot - Carboxypeptidase B2 antibody [13H4] (HRP) (ab14757)
Predicted band size : 50.8 kDa
Detection of carboxypeptidease B2 (TAFI) with ab14757 [13H4] in western blotting (non reducing conditions)
References for Anti-Carboxypeptidase B2 antibody [13H4] (HRP) (ab14757)
This product has been referenced in:
Acosta-Martin AE et al. Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm. PLoS One6:e28698 (2011).
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