Overview

  • Product name
    Anti-Carboxypeptidase H antibody
    See all Carboxypeptidase H primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase H
  • Specificity
    Ab11053 recognises carboxypeptidase H.
  • Tested applications
    Suitable for: ICC, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Carboxypeptidase H (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    LQQEDGISFE

  • Positive control
    • Secretory granules of the pancreatic islets, adrenal gland and pituitary gland.
  • General notes
    Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
IHC-Fr
IP
WB
  • Application notes
    ICC: 1/100 - 1/400.
    IHC-Fr: Use at an assay dependent dilution. Methanol fixation for this purpose is recommended.
    IP: Use at an assay dependent dilution. Pre-treatment with 0.1% w/v SDS and 1% v/v TritonX 100 required.
    WB: 1/500 - 1/1000. Predicted molecular weight: 53.2 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
    • Tissue specificity
      Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
    • Sequence similarities
      Belongs to the peptidase M14 family.
    • Cellular localization
      Nucleus and Secreted. Secretory granules of pancreatic islets, adrenal gland, pituitary and brain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Carboxypeptidase E antibody
      • Carboxypeptidase H antibody
      • Carboxypeptidase H precursor antibody
      • CarboxypeptidaseE antibody
      • CarboxypeptidaseH antibody
      • CBPE_HUMAN antibody
      • Cobalt stimulated chromaffin granule carboxypeptidase antibody
      • CPE antibody
      • Cph 1 antibody
      • CPH antibody
      • Enkephalin convertase antibody
      • Entephalin convertase antibody
      • Insulin granule associated carboxypeptidase antibody
      • Prohormone processing carboxypeptidase antibody
      • Prohormone-processing carboxypeptidase antibody
      see all

    References

    This product has been referenced in:
    • Parkinson D Carboxypeptidase H in bovine pituitary gland: soluble forms are not processed at the C-terminus. Mol Cell Endocrinol 86:221-33 (1992). Read more (PubMed: 1511790) »
    • Fiedorek FT & Parkinson D Carboxypeptidase H processing and secretion in rat clonal beta-cell lines. Endocrinology 131:1054-62 (1992). Read more (PubMed: 1505450) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab11053.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up