The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: 1/100 - 1/400.
IHC-Fr: Use at an assay dependent dilution. Methanol fixation for this purpose is recommended.
IP: Use at an assay dependent dilution. Pre-treatment with 0.1% w/v SDS and 1% v/v TritonX 100 required.
WB: 1/500 - 1/1000. Predicted molecular weight: 53.2 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRemoves residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
Tissue specificityIsoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
Sequence similaritiesBelongs to the peptidase M14 family.
Cellular localizationNucleus and Secreted. Secretory granules of pancreatic islets, adrenal gland, pituitary and brain.