Overview

  • Product name
    Anti-Carboxypeptidase H antibody
    See all Carboxypeptidase H primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase H
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Pig, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (GMRRRRRLQQ EDGISFEYHR YPELREALVS VWLQCTAISR IYTVGRSFEG) of Human Carboxypeptidase H (NP_001864).

  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
  • Tissue specificity
    Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
  • Sequence similarities
    Belongs to the peptidase M14 family.
  • Cellular localization
    Nucleus and Secreted. Secretory granules of pancreatic islets, adrenal gland, pituitary and brain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carboxypeptidase E antibody
    • Carboxypeptidase H antibody
    • Carboxypeptidase H precursor antibody
    • CarboxypeptidaseE antibody
    • CarboxypeptidaseH antibody
    • CBPE_HUMAN antibody
    • Cobalt stimulated chromaffin granule carboxypeptidase antibody
    • CPE antibody
    • Cph 1 antibody
    • CPH antibody
    • Enkephalin convertase antibody
    • Entephalin convertase antibody
    • Insulin granule associated carboxypeptidase antibody
    • Prohormone processing carboxypeptidase antibody
    • Prohormone-processing carboxypeptidase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Carboxypeptidase H with ab84631 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Carboxypeptidase H. Right - Merge.
  • Anti-Carboxypeptidase H antibody (ab84631) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling Carboxypeptidase H with ab84631 at 5µg/ml.

References

ab84631 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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