Recombinant
RabMAb

Anti-Carboxypeptidase M antibody [EPR8052] (ab150405)

Overview

  • Product name
    Anti-Carboxypeptidase M antibody [EPR8052]
    See all Carboxypeptidase M primary antibodies
  • Description
    Rabbit monoclonal [EPR8052] to Carboxypeptidase M
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Carboxypeptidase M aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: 293T cell lysate and human fetal kidney, fetal liver and placenta tissue lysates. IHC-P: Human hepatocellular carcinoma, endometrium and uterus tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR8052
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins.
    • Sequence similarities
      Belongs to the peptidase M14 family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Carboxypeptidase M antibody
      • CBPM_HUMAN antibody
      • CPM antibody
      • Renal carboxypeptidase antibody
      • Urinary carboxypeptidase B antibody
      see all

    Images

    • Anti-Carboxypeptidase M antibody [EPR8052] (ab150405) at 1/2500 dilution (purified) + Human placenta tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 51 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Carboxypeptidase M antibody [EPR8052] (ab150405) at 1/5000 dilution (purified)

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 51 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling Carboxypeptidase M with purified ab150405 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • All lanes : Anti-Carboxypeptidase M antibody [EPR8052] (ab150405) at 1/1000 dilution (unpurified)

      Lane 1 : 293T lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 51 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling Carboxypeptidase M with unpurified ab150405 at a dilution of 1/50.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling Carboxypeptidase M with unpurified ab150405 at a dilution of 1/50.

    References

    This product has been referenced in:
    • Lu D  et al. MicroRNA-146a promote cell migration and invasion in human colorectal cancer via carboxypeptidase M/src-FAK pathway. Oncotarget 8:22674-22684 (2017). WB, IHC-P ; Human . Read more (PubMed: 28186967) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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