Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Carboxypeptidase M aa 1-100 (N terminal). The exact sequence is proprietary.
WB: 293T cell lysate and human fetal kidney, fetal liver and placenta tissue lysates.
IHC-P: Human hepatocellular carcinoma, endometrium and uterus tissues.
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 51 kDa).
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for Flow Cyt or ICC/IF.
Specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. It is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase M antibody [EPR8052] (ab150405)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling Carboxypeptidase M with purified ab150405 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Carboxypeptidase M antibody [EPR8052] (ab150405)
All lanes : Anti-Carboxypeptidase M antibody [EPR8052] (ab150405) at 1/1000 dilution (unpurified)
Lane 1 : 293T lysate Lane 2 : Human fetal kidney lysate Lane 3 : Human fetal
liver lysate Lane 4 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Lu D et al. MicroRNA-146a promote cell migration and invasion in human colorectal cancer via carboxypeptidase M/src-FAK pathway. Oncotarget8:22674-22684 (2017).
Read more (PubMed: 28186967) »