Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab171176)

Overview

  • Product name
    Anti-Carboxypeptidase N subunit 2 antibody - N-terminal
    See all Carboxypeptidase N subunit 2 primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase N subunit 2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Carboxypeptidase N subunit 2 aa 20-50 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P22792

  • Positive control
    • HepG2 cell lysate; 293 cell lysate transiently transfected with the Carboxypeptidase N subunit 2 gene; Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab171176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.

Target

  • Function
    The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
  • Sequence similarities
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications
    Whether or not any Cys residues participate in intrachain bonds is unknown, but they do not form interchain disulfide bonds with the 50 kDa catalytic subunit.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACBP antibody
    • Carboxypeptidase N 83 kDa chain antibody
    • Carboxypeptidase N large subunit antibody
    • Carboxypeptidase N polypeptide 2 antibody
    • Carboxypeptidase N regulatory subunit antibody
    • Carboxypeptidase N subunit 2 [Precursor] antibody
    • Carboxypeptidase N subunit 2 antibody
    • carboxypeptidase N, polypeptide 2 antibody
    • Carboxypeptidase N, polypeptide 2, 83kD antibody
    • CPN 2 antibody
    • CPN2 antibody
    • CPN2_HUMAN antibody
    see all

Images

  • Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab171176) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 61 kDa

  • All lanes : Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab171176) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the Carboxypeptidase N subunit 2 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 61 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling Carboxypeptidase N subunit 2 with ab171176 at a 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171176 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab171176.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up