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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Ammonium Sulphate Precipitation
Purification notes ab85251 prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 61 kDa.
Function The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Sequence similarities Contains 12 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
Post-translational modifications Whether or not any Cys residues participate in intrachain bonds is unknown, but they do not form interchain disulfide bonds with the 50 kDa catalytic subunit.
Cellular localization Secreted.
Information by UniProt
Carboxypeptidase N 83 kDa chain antibody
Carboxypeptidase N large subunit antibody
Anti-Carboxypeptidase N subunit 2 antibody - N-terminal images
Western blot - Carboxypeptidase N subunit 2 antibody - N-terminal (ab85251)
Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab85251) at 1/50 dilution + Jurkat cell line lysate at 35 µg Predicted band size : 61 kDa Observed band size : 57 kDa ( why is the actual band size different from the predicted?) Additional bands at : 32 kDa,38 kDa,50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab85251)
has not yet been referenced specifically in any publications.
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