Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

Overview

  • Product nameAnti-Carboxypeptidase Y antibody [10A5B5]
    See all Carboxypeptidase Y primary antibodies
  • Description
    Mouse monoclonal [10A5B5] to Carboxypeptidase Y
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified)

  • Positive control
    • Yeast membranes
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab113685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionInvolved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
  • Sequence similaritiesBelongs to the peptidase S10 family.
  • Post-translational
    modifications
    Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).
  • Cellular localizationVacuole. Lysosome-like vacuoles.
  • Information by UniProt
  • Database links
    • Alternative names
      • carboxypeptidase C PRC1 antibody
      • Carboxypeptidase Y antibody
      • Carboxypeptidase YSCY antibody
      • CBPY_YEAST antibody
      • CPY antibody
      • LBC1 antibody
      • PRC1 antibody
      • Vacuolar carboxypeptidase Y antibody
      see all

    Anti-Carboxypeptidase Y antibody [10A5B5] images

    • All lanes : Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

      Lane 1 : Yeast whole cell lysate
      Lane 2 : Yeast membrane preparation

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 59 kDa

    References for Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

    This product has been referenced in:
    • Ast T  et al. A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins. J Cell Sci 127:3017-23 (2014). WB ; Saccharomyces cerevisiae . Read more (PubMed: 24849653) »
    • Manandhar SP  et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). Read more (PubMed: 23166297) »

    See all 4 Publications for this product

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