Overview

  • Product name
    Anti-Carboxypeptidase Y antibody
    See all Carboxypeptidase Y primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase Y
  • Tested applications
    Suitable for: WB, Dot blot, IP, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Carboxypeptidase Y [Baker's Yeast]

Properties

Applications

Our Abpromise guarantee covers the use of ab34636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 60 kDa).
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
  • Sequence similarities
    Belongs to the peptidase S10 family.
  • Post-translational
    modifications
    Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).
  • Cellular localization
    Vacuole. Lysosome-like vacuoles.
  • Information by UniProt
  • Database links
    • Alternative names
      • carboxypeptidase C PRC1 antibody
      • Carboxypeptidase Y antibody
      • Carboxypeptidase YSCY antibody
      • CBPY_YEAST antibody
      • CPY antibody
      • LBC1 antibody
      • PRC1 antibody
      • Vacuolar carboxypeptidase Y antibody
      see all

    Images

    • Anti-Carboxypeptidase Y antibody (ab34636) at 1/5000 dilution + Cellular extracts at 10 µg

      Secondary
      HRP goat anti-rabbit IgG at 1/4000 dilution

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 61 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Pfeiffer A  et al. A Complex of Htm1 and the Oxidoreductase Pdi1 Accelerates Degradation of Misfolded Glycoproteins. J Biol Chem 291:12195-207 (2016). Read more (PubMed: 27053108) »
    • Parsons HT  et al. Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis. Plant Physiol 159:12-26 (2012). WB . Read more (PubMed: 22430844) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Thank you for your reply.

    I would be happy to create a credit note/refund for your purchase of ab34636. In order to do this could you provide the PO used for that transaction? It is also very helpful for our internal testing if you could p...

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    Application
    Western blot
    Sample
    Pichia pastoris Cell lysate - whole cell (Yeast)
    Loading amount
    10 µg
    Specification
    Yeast
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Gaurav Agrawal

    Verified customer

    Submitted Mar 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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