Anti-Carboxypeptidase Y antibody (Biotin) (ab34663)

Overview

  • Product nameAnti-Carboxypeptidase Y antibody (Biotin)
    See all Carboxypeptidase Y primary antibodies
  • Description
    Rabbit polyclonal to Carboxypeptidase Y (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, Dot blot, ELISA, Immunomicroscopymore details
  • Immunogen

    Carboxypeptidase Y [Baker's Yeast]

  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab34663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
ELISA
Immunomicroscopy
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000. This antibody has been assayed against 1.0µg of Carboxypeptidase Y in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 60 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.
    • Sequence similaritiesBelongs to the peptidase S10 family.
    • Post-translational
      modifications
      Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).
    • Cellular localizationVacuole. Lysosome-like vacuoles.
    • Information by UniProt
    • Alternative names
      • carboxypeptidase C PRC1 antibody
      • Carboxypeptidase Y antibody
      • Carboxypeptidase YSCY antibody
      • CBPY_YEAST antibody
      • CPY antibody
      • LBC1 antibody
      • PRC1 antibody
      • Vacuolar carboxypeptidase Y antibody
      see all

    References for Anti-Carboxypeptidase Y antibody (Biotin) (ab34663)

    ab34663 has not yet been referenced specifically in any publications.

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