Mouse monoclonal [26/5/1] to Carcino Embryonic Antigen CEA
SpecificityAb26285 recognizes exclusively CEACAM5 transiently expressed on the cell surface of transfected BOSC cells. It can be used to distinguish CEACAM5 from all other CEACAM and probably all pregnancy specific glycoproteins molecules, namely CEACAM1 (BGP/CD66a), CEACAM3 (CGM1/CD66d), CEACAM4 (CGM7), CEACAM6 (NCA/CD66c), CEACAM7 (CGM2), CEACAM8 (CGM6/CD66b) and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 77 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Tissue specificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains.
Post-translational modificationsComplex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.