Anti-Carcino Embryonic Antigen CEA antibody [C6G9] (ab11330)

Overview

  • Product nameAnti-Carcino Embryonic Antigen CEA antibody [C6G9]
    See all Carcino Embryonic Antigen CEA primary antibodies
  • Description
    Mouse monoclonal [C6G9] to Carcino Embryonic Antigen CEA
  • SpecificityMonoclonal Anti-Human Carcinoembryonic Antigen (CEA) reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissues including colorectal, lung and breast tumors. It also stains medullary carcinomas of the thyroid. The product reacts strongly with the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. A weak reactivity is seen with normal colon mucosa and occasionally with bile canalicular and pancreatic acinar cells, but not with other tested normal tissues including term placenta.
  • Tested applicationsSuitable for: Dot blot, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CEA isolated from a human colon adenocarcinoma cell line.

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
WB
IHC-P
  • Application notesDot: 1/8000.
    IHC-P: 1/8000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    WB: 1/8000. Predicted molecular weight: 180 kDa.

    There is no cross reactivity with nonspecific cross-reacting antigen (NCA) present in granulocytes by immunoblotting, flow cytometry or immunohistochemical techniques.

    The antibody recognizes an epitope that is resistant to 30 minute oxidation by 1% sodium periodate solution in routine formalin-fixed, paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes enhances immunohistochemical staining of formalinfixed, paraffin-embedded tissue sections with the antibody.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
    • Tissue specificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
      Contains 7 Ig-like (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Carcinoembryonic antigen antibody
      • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
      • CD66e antibody
      • CEA antibody
      • Ceacam5 antibody
      • CEAM5_HUMAN antibody
      • DKFZp781M2392 antibody
      • Meconium antigen 100 antibody
      • OTTHUMP00000199032 antibody
      • OTTHUMP00000199033 antibody
      • OTTHUMP00000199034 antibody
      see all

    References for Anti-Carcino Embryonic Antigen CEA antibody [C6G9] (ab11330)

    This product has been referenced in:
    • Guo X  et al. In vitro evaluation of cancer-specific NF-kappaB-CEA enhancer-promoter system for 5-fluorouracil prodrug gene therapy in colon cancer cell lines. Br J Cancer 97:745-54 (2007). WB ; Human . Read more (PubMed: 17687334) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. For the following antibodies, epitope specificity is given per ISOBM classification and we have only following information: Ab10038 - Epitope specificity group IV, cross-reactive with NCA2. Ab10039 - Epitope specificity ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"