Anti-Carcino Embryonic Antigen CEA antibody [CEA31] (ab193372)

Overview

  • Product name
    Anti-Carcino Embryonic Antigen CEA antibody [CEA31]
    See all Carcino Embryonic Antigen CEA primary antibodies
  • Description
    Mouse monoclonal [CEA31] to Carcino Embryonic Antigen CEA
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IP, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human Carcino Embryonic Antigen CEA. Human colon carcinoma extract.

  • Positive control
    • MCF7 or 293T cells. Colon carcinoma; Human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab193372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

Use 1-2µg/500µg protein.

IHC-Fr Use a concentration of 1 - 2 µg/ml.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 76 kDa.

Target

  • Function
    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • Tissue specificity
    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling Carcino Embryonic Antigen CEA with ab193372 at 2 µg/ml.

References

ab193372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (HeLa, MCF-7, SW480 and Caco-2 clone (C2BBe1))
Permeabilization
No
Gating Strategy
The population gated was the viable single cells that showed fluorencense in the FITC-A channel. The flourochrome was present in the secondary antibody.
Specification
HeLa, MCF-7, SW480 and Caco-2 clone (C2BBe1)
Fixation
sodium azide
Username

Inês Pereira

Verified customer

Submitted Dec 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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