Anti-Carcino Embryonic Antigen CEA antibody [COL-1] (ab105364)

Overview

  • Product nameAnti-Carcino Embryonic Antigen CEA antibody [COL-1]
    See all Carcino Embryonic Antigen CEA primary antibodies
  • Description
    Mouse monoclonal [COL-1] to Carcino Embryonic Antigen CEA
  • SpecificityAb105364 stains specific regions of CEA. It reacts with colorectal adenocarcinomas but does not show any staining with polymorphonclear neutrophils and erythrocytes.
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were injected with the extract of colon carcinoma cells.

  • Positive control
    • Colon carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab105364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/50 - 1/100. Predicted molecular weight: 77 kDa.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP 1/50.

Target

  • FunctionCell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • Tissue specificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

Anti-Carcino Embryonic Antigen CEA antibody [COL-1] images

  • Overlay histogram showing LoVo cells stained with ab105364 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105364, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-Carcino Embryonic Antigen CEA antibody [COL-1] (ab105364)

This product has been referenced in:
  • Huber V  et al. Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape. Gastroenterology 128:1796-804 (2005). WB ; Human . Read more (PubMed: 15940614) »

See 1 Publication for this product

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