Anti-Carcino Embryonic Antigen CEA antibody [NCRC16 (AKA 161)] (ab22705)

Overview

  • Product name
    Anti-Carcino Embryonic Antigen CEA antibody [NCRC16 (AKA 161)]
    See all Carcino Embryonic Antigen CEA primary antibodies
  • Description
    Mouse monoclonal [NCRC16 (AKA 161)] to Carcino Embryonic Antigen CEA
  • Specificity
    Cross reacts with a similar epitope on NCA.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Colorectal cancer cell lines (Human).

  • Positive control
    • LS174 T cells, colon carcinoma IHC-P: FFPE human colon tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab22705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • Tissue specificity
    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

Anti-Carcino Embryonic Antigen CEA antibody [NCRC16 (AKA 161)] images

  • IHC image of Carcino Embryonic Antigen CEA staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22705, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab22705 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22705, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Carcino Embryonic Antigen CEA antibody [NCRC16 (AKA 161)] (ab22705)

This product has been referenced in:
  • Durrant LG  et al. Enhanced recognition of human colorectal tumour cells using combinations of monoclonal antibodies. Br J Cancer 60:855-60 (1989). Read more (PubMed: 2481485) »

See 1 Publication for this product

Product Wall

Thank you for submitting an Abreview of ab22705. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody for trying it in WB, we would like to follow up on thisto seeif w...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (recombinant protein)
Loading amount
0.02 µg
Specification
recombinant protein
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 13 2012

Application
ELISA
Sample
Human Purified protein (not sure)
Specification
not sure
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jan 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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