Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody, prediluted (ab15157)Image from Zhou J et al., World J Surg Oncol, 9:172. Fig 3.; doi: 10.1186/1477-7819-9-172
ab15157 staining Carcino Embryonic Antigen CEA in Human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was blocked with 10% goat serum in PBS for 30 minutes; antigen retrieval was by heat mediation in a citrate buffer (pH 6.0). Samples were incubated with primary antibody (1/10 in PBS containing 1% BSA) overnight at 4°C. A HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody. CEA was localized on the apical luminal surface of normal mucosa.
Zhou J et al. Dynamic expression of CEACAM7 in precursor lesions of gastric carcinoma and its prognostic value in combination with CEA. World J Surg Oncol9:172 (2011).
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