Carcino Embryonic Antigen (CEA) Human ELISA Kit (ab99992)

Overview

  • Product nameCarcino Embryonic Antigen (CEA) Human ELISA KitSee all Carcino Embryonic Antigen CEA kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.2 ng/ml
  • Range
    0.343 ng/ml - 250 ng/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 116.7 93% - 136%
    Serum 145.6 135% - 156%
    Plasma 103.8 82% - 124%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCarcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
  • Cellular localizationCell Membrane
  • Alternative names
    • Carcinoembryonic antigen
    • Carcinoembryonic antigen related cell adhesion molecule 5
    • CD66e
    • CD66e antigen
    • CEA
    • CEACAM 5
    • CEACAM5
    • DKFZp781M2392
    • Meconium antigen 100
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Carcino Embryonic Antigen (CEA) Human ELISA Kit images

  • Representative Standard Curve using ab99992.

  • Representative Standard Curve using ab99992.

Protocols

References for Carcino Embryonic Antigen (CEA) Human ELISA Kit (ab99992)

This product has been referenced in:
  • Chen S  et al. Increased miR-449a expression in colorectal carcinoma tissues is inversely correlated with serum carcinoembryonic antigen. Oncol Lett 7:568-572 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24396489) »

See 1 Publication for this product

Product Wall

CEA concentration of normal or lung cancer sera

Average Excellent 5/5 (Ease of Use)
Abreviews
Difference between normal group and lung adenocarcinoma (ADC) group was very clear.

But the absolute values measured by ab99992 were remarkably higher than clinical tests in hospital or CRO companies.

To make matters worse, when we increased the dilution factor (x2, x5, or x10 dilution), the absolute values of CEA also gradually increased.

Hence this kit can be used for only "relative quantification" studies.
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Abcam user community

Verified customer

Submitted Nov 05 2013

Thank you for your inquiry.

The capture antibody in this kit is supplied by an outside lab and unfortunately they were not willing to provide me with any other details about it. They did want to know if you were interested in it to follow up ...

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Thank you for your inquiry.

I can confirm that the kit should contain 8 ul of HRP-Streptavidin Concentrate (Item G). Please tell your customer to spin down the vial. The kit was ordered in November 2011 and we did change the amount of HRP-Str...

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Thank you for your new enquiry and continuous interest in our products.

I have conducted a search for you and we have FITC and PE-conjugated antibodies but not HRP-linked primary antibody against CEA.

However, we have two CEA ELISA ...

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Unfortunately there is no cross reactivity data available testing the Human CEA ELISA Kit ab99992 with mouse. If it helps though, the capture antibody is a mouse monoclonal and reacts with the protein determinant belonging to the “gold” epitope group 4...

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At the moment we do not sell kit components separately, this is because that we only produce enough reagents for use with the kit and do not have any spare to sell separately. We may in future add the antibody in its own right but for now it can only ...

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Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"