Overview

  • Product name
  • Description
    Rabbit polyclonal to CARD10
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids at the C terminus of human CARD10 (Genbank accession No.AAK26165).

  • Positive control
    • EL4 cell lysate can be used as a positive control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 116 kDa.Can be blocked with Human CARD10 peptide (ab39737).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Activates NF-kappa-B via BCL10 and IKK.
  • Tissue specificity
    Detected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.
  • Sequence similarities
    Contains 1 CARD domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bcl10 binding protein and activator of NFKB antibody
    • BIMP1 antibody
    • CAR10_HUMAN antibody
    • CARD 10 antibody
    • CARD containing MAGUK 3 protein antibody
    • CARD-containing MAGUK 3 antibody
    • CARD-containing MAGUK protein 3 antibody
    • CARD10 antibody
    • Carma 3 antibody
    • CARMA3 antibody
    • Caspase Recruitment Domain family member 10 antibody
    • Caspase recruitment domain-containing protein 10 antibody
    see all

Anti-CARD10 antibody images



  • Predicted band size : 116 kDa
    Western Blot of A20 cell lysate labeling CARD10 with Anti-CARD10 antibody (ab36839) at 5µg/ml.
  • Immunofluorescence of CARD10 in EL4 cells using ab36839 at 20 ug/ml.

References for Anti-CARD10 antibody (ab36839)

This product has been referenced in:
  • Hicks SD & Miller MW Effects of ethanol on transforming growth factor ?1-dependent and -independent mechanisms of neural stem cell apoptosis. Exp Neurol 229:372-80 (2011). WB, ICC ; Mouse . Read more (PubMed: 21419121) »

See 1 Publication for this product

Product Wall

Thank you for contacting us regarding this. I could not find any historical data showing that we had offered an anti-Card10 or anti-CARM3 raised in sheep. I hope this information is helpful. Please let me know if have any questions.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate Buffer pH 6
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Buffered Formalin
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Verified customer

Submitted Sep 30 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Sep 25 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreatic tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6
Permeabilization
No
Specification
pancreatic tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

Submitted Sep 09 2015

Thank you for your enquiry. I have enquired at the originator of the product and they have confirmed that the immunogen for this antibody has no homology with CARD11 and a BLAST search does not yield proteins that large, so cross-reaction with line...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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