The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 0.1 - 1 µg/ml.
IP: Use 3 - 5µg/extract of 107 cells.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 116 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActivates NF-kappa-B via BCL10 and IKK.
Tissue specificityDetected in adult heart, kidney and liver; lower levels in intestine, placenta, muscle and lung. Also found in fetal lung, liver and kidney.