Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
Caspase recruitment domain containing protein 11 antibody
Caspase recruitment domain family member 11 antibody
Caspase recruitment domain-containing protein 11 antibody
Western blot - CARD11 antibody (ab91463)
All lanes : Anti-CARD11 antibody (ab91463) at 0.1 µg/ml
Lane 1 : Ramos cell lysate at 50 µg Lane 2 : Ramos cell lysate at 15 µg Lane 3 : Ramos cell lysate at 5 µg Lane 4 : K562 cell lysate at 50 µg
Predicted band size: 133 kDa
Immunoprecipitation - CARD11 antibody (ab91463)
Sample: whole cell lysate from Ramos cells (1 mg for IP, 20% of IP loaded) . Antibody: ab91463 at 1 µg/ml for WB and at 10 µg/mg lysate for IP. Detection: chemiluminescence with exposure times of 10 seconds.