Overview

  • Product nameAnti-CARD11 antibody
    See all CARD11 primary antibodies
  • Description
    Rabbit polyclonal to CARD11
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 275-325 of Human CARD11 (NP_115791.3).

  • Positive control
    • Ramos cell lysate and K562 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23129749
WB 1/2000 - 1/10000. Predicted molecular weight: 133 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
  • Sequence similaritiesContains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
  • Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610008L17Rik antibody
    • 2410011D02Rik antibody
    • Bcl10 interacting maguk protein 3 antibody
    • BIMP 3 antibody
    • BIMP3 antibody
    • CAR11_HUMAN antibody
    • CARD 11 antibody
    • CARD containing MAGUK protein 3 antibody
    • Card maguk protein 1 antibody
    • CARD-containing MAGUK protein 1 antibody
    • CARD11 antibody
    • CARD11 protein antibody
    • Carma 1 antibody
    • CARMA1 antibody
    • Caspase recruitment domain containing protein 11 antibody
    • Caspase recruitment domain family member 11 antibody
    • Caspase recruitment domain-containing protein 11 antibody
    • MGC133069 antibody
    see all

Anti-CARD11 antibody images

  • All lanes : Anti-CARD11 antibody (ab91463) at 0.1 µg/ml

    Lane 1 : Ramos cell lysate at 50 µg
    Lane 2 : Ramos cell lysate at 15 µg
    Lane 3 : Ramos cell lysate at 5 µg
    Lane 4 : K562 cell lysate at 50 µg


    Predicted band size : 133 kDa
  • Sample: whole cell lysate from Ramos cells (1 mg for IP, 20% of IP loaded) .
    Antibody: ab91463 at 1 µg/ml for WB and at 10 µg/mg lysate for IP.
    Detection: chemiluminescence with exposure times of 10 seconds.

References for Anti-CARD11 antibody (ab91463)

This product has been referenced in:
  • Snow AL  et al. Congenital B cell lymphocytosis explained by novel germline CARD11 mutations. J Exp Med 209:2247-61 (2012). ICC/IF ; Human . Read more (PubMed: 23129749) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"