Overview

  • Product nameAnti-CARD11 antibody [EPR3177]
    See all CARD11 primary antibodies
  • Description
    Rabbit monoclonal [EPR3177] to CARD11
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues on the C terminus of Human CARD11.

  • Positive control
    • K562 and Ramos cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa).
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionInvolved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
    • Tissue specificityDetected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
    • Sequence similaritiesContains 1 CARD domain.
      Contains 1 guanylate kinase-like domain.
      Contains 1 PDZ (DHR) domain.
    • Post-translational
      modifications
      Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
    • Cellular localizationCytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0610008L17Rik antibody
      • 2410011D02Rik antibody
      • Bcl10 interacting maguk protein 3 antibody
      • BIMP 3 antibody
      • BIMP3 antibody
      • CAR11_HUMAN antibody
      • CARD 11 antibody
      • CARD containing MAGUK protein 3 antibody
      • Card maguk protein 1 antibody
      • CARD-containing MAGUK protein 1 antibody
      • CARD11 antibody
      • CARD11 protein antibody
      • Carma 1 antibody
      • CARMA1 antibody
      • Caspase recruitment domain containing protein 11 antibody
      • Caspase recruitment domain family member 11 antibody
      • Caspase recruitment domain-containing protein 11 antibody
      • MGC133069 antibody
      see all

    Anti-CARD11 antibody [EPR3177] images

    • All lanes : Anti-CARD11 antibody [EPR3177] (ab109310) at 1/1000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : Ramos cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 133 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    References for Anti-CARD11 antibody [EPR3177] (ab109310)

    ab109310 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109310.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"