Recombinant Anti-Carma 1/CARD11 antibody [EPR3177] (ab109310)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3177] to Carma 1/CARD11
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Carma 1/CARD11 antibody [EPR3177]
See all Carma 1/CARD11 primary antibodies -
Description
Rabbit monoclonal [EPR3177] to Carma 1/CARD11 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562 and Ramos cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3177 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa). |
Target
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Function
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. -
Tissue specificity
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsPhosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling. -
Cellular localization
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 84433 Human
- Entrez Gene: 108723 Mouse
- Omim: 607210 Human
- SwissProt: Q9BXL7 Human
- SwissProt: Q8CIS0 Mouse
- Unigene: 648101 Human
- Unigene: 46187 Mouse
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Alternative names
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
see all
Images
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All lanes : Anti-Carma 1/CARD11 antibody [EPR3177] (ab109310) at 1/1000 dilution
Lane 1 : K562 cell lysates
Lane 2 : Ramos cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 133 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109310 has not yet been referenced specifically in any publications.