Recombinant
RabMAb

Anti-CARD11 antibody [EPR3177] (ab109310)

Overview

  • Product name
    Anti-CARD11 antibody [EPR3177]
    See all CARD11 primary antibodies
  • Description
    Rabbit monoclonal [EPR3177] to CARD11
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues on the C terminus of Human CARD11.

  • Positive control
    • K562 and Ramos cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
    • Tissue specificity
      Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
    • Sequence similarities
      Contains 1 CARD domain.
      Contains 1 guanylate kinase-like domain.
      Contains 1 PDZ (DHR) domain.
    • Post-translational
      modifications
      Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
    • Cellular localization
      Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • 0610008L17Rik antibody
      • 2410011D02Rik antibody
      • Bcl10 interacting maguk protein 3 antibody
      • BIMP 3 antibody
      • BIMP3 antibody
      • CAR11_HUMAN antibody
      • CARD 11 antibody
      • CARD containing MAGUK protein 3 antibody
      • Card maguk protein 1 antibody
      • CARD-containing MAGUK protein 1 antibody
      • CARD11 antibody
      • CARD11 protein antibody
      • Carma 1 antibody
      • CARMA1 antibody
      • Caspase recruitment domain containing protein 11 antibody
      • Caspase recruitment domain family member 11 antibody
      • Caspase recruitment domain-containing protein 11 antibody
      • MGC133069 antibody
      see all

    Images

    • All lanes : Anti-CARD11 antibody [EPR3177] (ab109310) at 1/1000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : Ramos cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 133 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    References

    ab109310 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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