The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 114 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
FunctionPlays a role in the promotion of apoptosis.
Tissue specificityIsoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115537 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-CARD12 antibody (ab115537)
has not yet been referenced specifically in any publications.
Publishing research using ab115537? Please let us know so that we can cite the reference in this datasheet.
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