Overview

  • Product nameAnti-CARD15 antibody [26mNOD2]
    See all CARD15 primary antibodies
  • Description
    Rat monoclonal [26mNOD2] to CARD15
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse CARD15.

  • Positive control
    • Mouse Lymph Node tissue; Mouse CARD15 Transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number26mNOD2
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 115 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionInduces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
  • Tissue specificityMonocytes-specific.
  • Involvement in diseaseDefects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
    Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
  • Sequence similaritiesContains 2 CARD domains.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACUG antibody
    • Arthrocutaneouveal granulomatosis antibody
    • BLAU antibody
    • CARD15 antibody
    • Caspase recruitment domain family, member 15 antibody
    • Caspase recruitment domain protein 15 antibody
    • Caspase recruitment domain-containing protein 15 antibody
    • CD antibody
    • CLR16.3 antibody
    • IBD1 antibody
    • Inflammatory bowel disease protein 1 antibody
    • LRR containing protein antibody
    • NLR family, CARD domain containing 2 antibody
    • NLRC2 antibody
    • NOD like receptor C2 antibody
    • NOD2 antibody
    • NOD2 protein antibody
    • NOD2_HUMAN antibody
    • NOD2B antibody
    • nucleotide binding oligomerization domain 2 antibody
    • Nucleotide binding oligomerization domain containing 2 antibody
    • Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody
    • Nucleotide-binding oligomerization domain-containing protein 2 antibody
    • PSORAS1 antibody
    see all

Anti-CARD15 antibody [26mNOD2] images

  • ab124348, at 10ug/ml, staining CARD15 in formalin-fixed, paraffin-embedded Mouse lymph node tissue by Immunohistochemistry.
  • All lanes : Anti-CARD15 antibody [26mNOD2] (ab124348) at 2 µg/ml

    Lane 1 : 293T cell control lysate
    Lane 2 : 293T cell lysate transfected with Mouse CARD15

    Lysates/proteins at 200000 cells per lane.

    Secondary
    Polyclonal anti Rat secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 115 kDa

References for Anti-CARD15 antibody [26mNOD2] (ab124348)

ab124348 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"