Overview

  • Product name
  • Description
    Rabbit polyclonal to CARD15
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2.

  • Positive control
    • HeLa cell lysate.
  • General notes


    This antibody has no cross-reaction with NOD1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    This antibody has no cross-reaction with NOD1.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 115 kDa).
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
  • Tissue specificity
    Monocytes-specific.
  • Involvement in disease
    Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
    Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
  • Sequence similarities
    Contains 2 CARD domains.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACUG antibody
    • Arthrocutaneouveal granulomatosis antibody
    • BLAU antibody
    • CARD15 antibody
    • Caspase recruitment domain family, member 15 antibody
    • Caspase recruitment domain protein 15 antibody
    • Caspase recruitment domain-containing protein 15 antibody
    • CD antibody
    • CLR16.3 antibody
    • IBD1 antibody
    • Inflammatory bowel disease protein 1 antibody
    • LRR containing protein antibody
    • NLR family, CARD domain containing 2 antibody
    • NLRC2 antibody
    • NOD like receptor C2 antibody
    • NOD2 antibody
    • NOD2 protein antibody
    • NOD2_HUMAN antibody
    • NOD2B antibody
    • nucleotide binding oligomerization domain 2 antibody
    • Nucleotide binding oligomerization domain containing 2 antibody
    • Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody
    • Nucleotide-binding oligomerization domain-containing protein 2 antibody
    • PSORAS1 antibody
    see all

Images

  • Anti-CARD15 antibody (ab36836) at 2 µg/ml + HeLa cell lysate

    Predicted band size : 115 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab36836 at 10µg/ml staining CARD15 in Hela cells by Immunocytochemistry.

  • IHC image of ab36836 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36836, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence of NOD2 in HeLa cells using ab36836 at 20 ug/ml.

References

This product has been referenced in:
  • Wang B  et al. Increased MIR31HG lncRNA expression increases gefitinib resistance in non-small cell lung cancer cell lines through the EGFR/PI3K/AKT signaling pathway. Oncol Lett 13:3494-3500 (2017). Read more (PubMed: 28529576) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 22 2014

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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