Ab47918 recognizes CARD15, a 1040 amino acid protein. Ab47918 also recognizes CARD15 alternatively spliced and mutated forms which contain the peptide immunogen sequence including CARD15 short, a 273 amino acid protein.
Human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Human colon tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/1000 - 1/5000.
IP: 1/50 - 1/200.
WB: 1/1000 - 1/2000. Predicted molecular weight: 115 kDa. CARD15 produces, by alternative splicing, 13 different transcripts, all with introns, putatively encoding 13 different protein isoforms. Therefore, the molecular weights observed for CARD15 on Western blot may vary. Users are encoraged to go to the NCBI ACE View Data Base for more information about CARD15 isoforms: http://www.ncbi.nih.gov/IEB/Research/Acembly/
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
Involvement in disease
Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash. Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
Nucleotide-binding oligomerization domain-containing protein 2 antibody
Anti-CARD15 antibody images
Western blot - CARD15 antibody (ab47918)
All lanes : Anti-CARD15 antibody (ab47918) at 1/2000 dilution
Lane 1 : PPC-1 (prostate carcinoma) Lane 2 : NCI-H226(lung carcinoma) Lane 3 : HOP-92 Lane 4 : SF-268 (glioblastoma) Lane 5 : UO-31 (renal/kidney carcinoma) Lane 6 : A498 (renal/kidney carcinoma) Lane 7 : SK-MEL-5 Lane 8 : SK-MEL-28 (melanoma) Lane 9 : MCF-7 (breast carcinoma) Lane 10 : Lymphoma. Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.